Microadaption of hemadsorption inhibition for neutralization tests with pig hemagglutinating encephalomyelitis virus.

Effect of influenza anti-neuraminidase antibody on virus neutralization.

Antibody to influenza virus neuraminidase participates in tissue culture neutralization tests in which inhibition of hemadsorption is the end point. Presumably, by inhibiting virus release it functions additively with anti-hemagglutinin in reducing total virus replication.

Comparison of Micro Neutralization Test with Hemadsorption-Inhibition Test, and Immunoflrorescence Technique for Detecting Mumps Antibodies

Hemolysis-in-gel and neutralization tests for determination of antibodies to mumps virus.

A hemolysis-in-gel test for the demonstration of antibodies to mumps virus is described. The results were compared with those of neutralization tests using a modified microtechnique. In the neutralization test viral replication was demonstrated by the hemadsorption of guinea pig erythrocytes, the visibility of which could be further enhanced by the use of o-tolidine. Good correlation was found ...

A Sensitive Neutralization Assay for Influenza C Viruses Based on the Acetylesterase Activity HEF Glycoprotein

Influenza C virus possesses specific neuraminate-O-acetylesterase as a receptor-destroying function. This enzymatic activity of the viral glycoprotein HEF (Hemagglutinin, esterase activity and fusion factor) can be visualized in situ by the use of distinct color substrates. Hereby the localization, as well as the quantity of synthesized HEF protein is detectable. We further developed the estera...

Analysis of the properties of neutralizing monoclonal antibodies against the hemagglutinating encephalomyelitis virus and inhibition of HEV infection by specific MAb.

Eleven monoclonal antibodies (MAbs) that were reactive against the hemagglutinating encephalomyelitis virus (HEV), as seen in the enzyme-linked immunosorbent assay, were obtained. All of these MAbs showed neutralizing activity (1:20,000 to 1:800,000) against the 67N strain of HEV. They also showed hemagglutination inhibition activity (1:400 to 1:409,600). Western blotting tests revealed that al...